FastQCFastQC Report
Sat 18 Mar 2023
SRR4895572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388380
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10740.27653329213656724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6810.17534373551676194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA6650.17122405891137546No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6150.15835006951954272No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC5150.13260209073587723No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT4880.12565013646428755No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA4300.11071630876976156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA4240.10917143004274164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGGGTGGCTCACGCCT4000.10299191513466192No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT3910.10067459704413202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT88550.0139.205221
GGTATCA89350.0137.341678
GTGGTAT89700.0137.28726
TGGTATC90000.0136.74967
AGTGGTA90100.0136.597825
GTATCAA89700.0136.484829
CAGTGGT91000.0135.167774
AGCAGTG91450.0134.790822
GCAGTGG92950.0132.46073
GTATCCA750.001536318738.3858579
CGCGAAA602.0008883E-1128.81313390-94
GTACGAC255.370558E-428.7982930-34
TCAACGC89200.028.25685310-14
ATCAACG89200.028.16002710-14
AGAGTAC89350.028.11275115-19
CAGAGTA89600.028.06644215-19
GTACATG90100.027.90046320-24
CAACGCA90250.027.89620210-14
TACATGG90000.027.8354820-24
GCAGAGT90700.027.74192615-19
Produced by FastQC (version 0.11.5)