FastQCFastQC Report
Sat 18 Mar 2023
SRR4895571_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895571_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525761
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTGC527000.0143.546772
AATGTTG528350.0143.181
TGTTGCA128650.0142.378163
GTTGCAT42650.0142.032534
TGTTGCG144600.0141.599383
GTTGCGA32450.0141.005864
TGTTGCT154050.0140.992553
TGCATAG4600.0140.760066
GTTGCGT49600.0140.40694
GTTGCAG41150.0140.39144
GTTGCTA39750.0140.268254
TGTTGCC108400.0139.640463
GTTGCAA34400.0139.077854
TTGCATA12050.0138.514655
TTGCGTA14450.0138.411355
GTTGCTG51000.0138.104344
GTTGCGG48950.0137.27424
TTGCGAG10850.0137.257265
TGCGGAT4200.0137.036256
TTGCATG15550.0136.948125
Produced by FastQC (version 0.11.5)