FastQCFastQC Report
Sat 18 Mar 2023
SRR4895571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525761
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25320.48158764153293987No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG16340.3107876012104359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC9220.1753648520905887No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC5940.11297909126009728No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCATGCCT5650.10746327704032821No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT5550.10556127213695957No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA5430.10327886625291718No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC5390.10251806429156973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT101850.0135.829861
GTGGTAT102000.0135.526926
GGTATCA102250.0135.195568
TGGTATC102450.0134.861397
AGTGGTA103200.0134.090485
GTATCAA103200.0133.951029
CAGTGGT104050.0132.995074
AGCAGTG104750.0132.138172
GCAGTGG106900.0129.480583
ACGTCGC304.610089E-528.803654110-114
TCAACGC102750.027.42579510-14
AGAGTAC102200.027.34963215-19
ATCAACG102750.027.32774410-14
CAGAGTA102500.027.28362715-19
CAACGCA103750.027.18919610-14
GTACATG103300.027.05115120-24
TACATGG103650.026.98758720-24
GCAGAGT104250.026.86704815-19
AACGCAG105300.026.80264310-14
CGCAGAG104200.026.79706215-19
Produced by FastQC (version 0.11.5)