FastQCFastQC Report
Sat 18 Mar 2023
SRR4895570_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895570_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727450
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTC1730300.0143.657622
AATCCGT1730350.0143.653491
TCCGTCG506200.0142.800233
TCCGTCA430500.0142.77493
TCCGTCT491450.0141.96223
CCGTCTA134350.0141.913224
CCGTCGT176100.0141.851854
CCGTCGA130400.0141.798024
CCGTCAG124950.0141.764144
CCGTCTG144600.0140.411714
TCCGTCC321050.0140.331453
CCGTCAT155200.0140.232384
CGTCGAT47400.0139.796395
CCGTCGG151450.0139.761664
CGTCGTA51750.0139.723795
CGTCAGT44450.0139.686255
CGTCTAA38950.0139.646155
CCGTCAA120150.0138.92454
CGTCGGA39350.0138.592295
CGTCGAG38800.0138.517145
Produced by FastQC (version 0.11.5)