FastQCFastQC Report
Sat 18 Mar 2023
SRR4895570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727450
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73090.4231092072129439No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG30650.1774291585863556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT25650.14848476077455208No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC22590.13077078931372832No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC20530.11884569741526527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGTGCAGTGGCTCA18570.10749949347303829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT314350.0138.530141
GTGGTAT316400.0137.45236
GGTATCA316250.0137.449228
TGGTATC318450.0136.590067
GTATCAA318550.0136.45689
AGTGGTA320450.0135.804935
AGCAGTG322550.0134.986052
CAGTGGT323000.0134.911044
GCAGTGG335100.0129.973593
TCAACGC316300.028.1407810-14
ATCAACG316100.028.04930310-14
AGAGTAC318350.027.86510815-19
TACATGG318900.027.7957620-24
CAGAGTA318950.027.79012515-19
GTACATG321150.027.60998720-24
GCAGAGT323000.027.45058415-19
CAACGCA326600.027.29296910-14
AGTACAT324150.027.28783620-24
GAGTACA317600.027.24771920-24
ACATGGG323450.027.16440220-24
Produced by FastQC (version 0.11.5)