FastQCFastQC Report
Sat 18 Mar 2023
SRR4895569_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895569_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887759
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACAC891950.0143.216222
AAGGACA893350.0142.991781
GGACACT260950.0142.901213
GACACTG80850.0142.825914
GACACGT79550.0142.44674
GGACACG241500.0142.34463
GACACTA70050.0142.250444
GACACAT80750.0142.02274
GGACACA236050.0141.790683
GACACTT85750.0141.124974
GACACGA64250.0140.086474
GACACAG73300.0139.77134
GGACACC162200.0139.369373
GACACGG75300.0138.925324
GACACCT41350.0137.803964
ACACTAG22350.0137.777485
ACACGTA26300.0137.054725
ACACTGT31550.0137.052545
GACACCG39200.0136.185184
ACACGAT24750.0135.463645
Produced by FastQC (version 0.11.5)