FastQCFastQC Report
Sat 18 Mar 2023
SRR4895569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences887759
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48960.5515010267426181No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG23180.26110689950763666No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC17620.19847728944454518No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC16360.18428424831513956No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT12920.14553499316819093No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA12690.14294419994615656No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC12360.1392269748884551No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA10930.12311899963841537No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA10570.11906384503001378No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT10320.116247765440846No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG10300.11602247907371258No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA10290.11590983589014586No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT10020.11286846993384465No Hit
GTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAG9890.1114041085474774No Hit
GGCAGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCG9650.10870067214187634No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA9440.10633516528697541No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT9370.10554666300200842No Hit
GCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGT9230.10396965843207447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTTTCTTGCTGCAGCAACGC9220.10385701524850777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT147900.0138.721771
GGTATCA149000.0137.65298
GTGGTAT149400.0137.284356
TGGTATC150550.0136.283467
GTATCAA150600.0136.238249
CAGTGGT153350.0133.842034
AGCAGTG153550.0133.664292
AGTGGTA154100.0133.143925
GCAGTGG157650.0130.279453
TCAACGC149250.028.27524410-14
ATCAACG149050.028.2069610-14
CAACGCA149700.028.19024810-14
AGAGTAC149550.028.08663215-19
CAGAGTA149850.028.03040115-19
TACATGG150650.027.92669320-24
GTACATG151450.027.74114820-24
GCAGAGT151600.027.71632415-19
CGCAGAG151550.027.68747715-19
AGTACAT151200.027.68226820-24
GAGTACA149700.027.28638320-24
Produced by FastQC (version 0.11.5)