FastQCFastQC Report
Sat 18 Mar 2023
SRR4895568_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895568_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586143
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTA1587400.0143.805181
CGAGTAA1587900.0143.75992
GAGTAAG497750.0143.098983
GAGTAAT531700.0143.083943
GAGTAAC199150.0142.766753
AGTAACG59250.0140.765234
GAGTAAA374000.0140.47893
AGTAATG176700.0140.339234
AGTAATA156750.0139.469924
AGTAAGT157600.0139.356964
AGTAAGA139050.0139.31714
AGTAAGG168000.0138.954284
AGTAATC65600.0138.876974
AGTAAGC47300.0138.446064
AGTAACT44200.0137.899124
GTAAGTA48550.0137.549535
GTAATCG21350.0136.844865
GTAACGT19100.0136.387825
GTAATAG52700.0136.003195
AGTAACC58900.0135.615114
Produced by FastQC (version 0.11.5)