FastQCFastQC Report
Sat 18 Mar 2023
SRR4895568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586143
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68280.430478210350517No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG30760.19392955111865703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC28590.1802485652302472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT327600.0139.072221
GGTATCA329700.0138.099298
GTGGTAT329900.0138.037386
TGGTATC331150.0137.55987
GTATCAA332900.0136.879919
CAGTGGT334150.0136.281714
AGCAGTG335500.0135.60432
AGTGGTA336750.0135.122625
GCAGTGG340700.0133.598023
CGTGGTA1351.0946678E-842.6518065
TCAACGC329200.028.26087210-14
ATCAACG328700.028.25130710-14
AGAGTAC332550.027.96752215-19
CAGAGTA333550.027.87935615-19
TACATGG333450.027.82752620-24
GTACATG333650.027.8151620-24
AGTACAT334550.027.70590620-24
CAACGCA336900.027.65341210-14
GCAGAGT337700.027.51117315-19
GAGTACA332050.027.38985820-24
Produced by FastQC (version 0.11.5)