FastQCFastQC Report
Sat 18 Mar 2023
SRR4895567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386591
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71480.5155088991634881No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG39740.28660217757074724No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC30890.22277657939507758No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC21010.15152269126223955No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC18640.13443041242875514No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA17110.12339615647296137No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT16640.1200065484342535No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC16240.11712177563535318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG14570.10507784919994431No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC14090.10161612184126394No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA13980.10082280932156634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT255200.0139.266921
GGTATCA255000.0138.957038
GTGGTAT256000.0138.498586
GTATCAA256700.0138.008749
TGGTATC257200.0137.880377
AGTGGTA259600.0136.550225
AGCAGTG261400.0135.798432
CAGTGGT261550.0135.752324
GCAGTGG270450.0131.30753
TCAACGC255850.028.22233610-14
ATCAACG255350.028.19867310-14
AGAGTAC256300.027.97620615-19
CAGAGTA257100.027.87795415-19
CAACGCA259800.027.77108210-14
TACATGG258150.027.75721520-24
GTACATG258950.027.73818220-24
AGTACAT259750.027.58623920-24
GAGTACA256600.027.42552420-24
CGCAGAG262200.027.40707815-19
GCAGAGT262200.027.39609715-19
Produced by FastQC (version 0.11.5)