FastQCFastQC Report
Sat 18 Mar 2023
SRR4895566_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895566_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163455
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC2550.15600624024961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTA151.2671339E-4143.866628
CATATAG100.0072536655143.866628
CGTCTAA100.0072536655143.866628
CGATTAG202.2098648E-6143.866628
CCGCTAG202.2098648E-6143.866627
CTACGAT100.0072536655143.866628
CGCTAGA100.0072536655143.866628
CGTACTA100.0072536655143.866628
CCGACTG100.0072536655143.866627
CCGACAT100.0072536655143.866627
CTGATAC100.0072536655143.866628
CAGAGTA100.0072536655143.866628
CGATATA253.8748112E-8143.866628
AACTCAC163850.0143.515411
ACTCACC164000.0143.384142
CTCACCG59000.0143.257023
TCACCGA14500.0142.874444
TCACCTG16100.0142.526254
TCACCGG17900.0142.259174
CTCACCT47700.0142.056983
Produced by FastQC (version 0.11.5)