FastQCFastQC Report
Sat 18 Mar 2023
SRR4895566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163455
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7630.46679514239393105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6820.41724021902052555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5040.30834174543452325No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4700.28754091340124194No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC4100.2508335627542749No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT3020.18476033158973418No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG2730.1670184454437001No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC2340.14315866752317152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC2220.13581719739377812No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC2040.12480499219968799No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT2000.12235783548989018No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT1980.12113425713499129No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA1930.11807531124774402No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT1900.11623994371539569No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA1810.1107338411183506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT30450.0138.15591
GTGGTAT30700.0136.436256
AGCAGTG30850.0136.131072
CAGTGGT30950.0135.799264
GGTATCA30900.0135.553188
AGTGGTA31000.0135.11595
GTATCAA31150.0134.465269
TGGTATC31200.0134.249777
GCAGTGG32050.0131.258883
GAATGCG200.00623966328.84051595-99
ACGATCA200.00625088128.829912120-124
CGCGAAA255.344987E-428.8193290-94
GTTCTCG255.36802E-428.798153110-114
AGAGTAC30400.028.03001415-19
TCAACGC30800.027.94638610-14
TACATGG30750.027.71605920-24
ATCAACG30900.027.66961510-14
CAGAGTA30750.027.66416215-19
GTACATG31250.027.41080920-24
AGTACAT31100.027.31155820-24
Produced by FastQC (version 0.11.5)