FastQCFastQC Report
Sat 18 Mar 2023
SRR4895564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1104697
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45770.4143217551962212No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT43960.39793717191229816No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG35470.321083518829145No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC26520.24006582800532636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC24390.2207845228148533No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT16180.1464655013999314No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGCAATAGATATAGTACC13280.12021395912182255No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC13240.1198518688835038No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGCCCCTCATTTACATAAAT12770.11559730858325859No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA11810.10690714286360875No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC11570.1047346014336963No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT11090.10038951857387138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT245400.0138.487841
GGTATCA246850.0137.60188
TGGTATC247200.0137.406977
GTGGTAT247150.0137.347416
GTATCAA247700.0137.100569
AGTGGTA249550.0135.997685
CAGTGGT251850.0135.069994
AGCAGTG251750.0134.96612
GCAGTGG254550.0133.481493
TCAACGC247200.028.11602810-14
ATCAACG247150.028.04018810-14
AGAGTAC248100.027.93357815-19
CAGAGTA249250.027.81047215-19
CAACGCA250250.027.78485910-14
TACATGG249150.027.7822120-24
GTACATG250950.027.61735720-24
AGTACAT251600.027.54600720-24
GCAGAGT251700.027.50545915-19
CGCAGAG252800.027.4370215-19
GAGTACA248300.027.32069620-24
Produced by FastQC (version 0.11.5)