FastQCFastQC Report
Sat 18 Mar 2023
SRR4895563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105555
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG60620.5483218835788359No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT43590.3942816051666358No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42510.3845127560365608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC32900.2975880892402459No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT23150.2093970901492915No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA19420.17565837972782902No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC16480.14906540154040276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG12970.11731664186765924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT264750.0136.996431
GGTATCA267200.0135.771978
GTGGTAT267500.0135.592796
GTATCAA268250.0135.160059
TGGTATC269250.0134.845157
AGTGGTA270250.0134.213065
AGCAGTG271450.0133.615052
CAGTGGT272800.0133.116784
GCAGTGG277650.0130.579533
ATCAACG266900.027.74037410-14
TCAACGC268000.027.71781310-14
AGAGTAC268950.027.5396315-19
CAGAGTA269300.027.46642715-19
TACATGG270050.027.41741620-24
CAACGCA271450.027.3814410-14
GTACATG271550.027.22885520-24
GCAGAGT273400.027.05453115-19
AACGCAG274700.027.03653110-14
CGCAGAG273500.027.01832615-19
AGTACAT273500.026.97155420-24
Produced by FastQC (version 0.11.5)