FastQCFastQC Report
Sat 18 Mar 2023
SRR4895562_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895562_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636299
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGAACA639750.0143.089891
GAACAAT218000.0142.986043
GGAACAA645500.0141.815262
GAACAAG198050.0140.971133
GAACAAC86350.0138.211913
AACAATG71600.0138.150074
ACAATGT25350.0135.647355
AACAAGT61950.0135.400014
ACAATGA18750.0135.052555
ACAATGG24500.0134.187335
ACAAGTA20150.0133.880585
CAATGAG5850.0132.809566
CAATATG9100.0132.809566
ACAAGTG19600.0132.131965
GAACAAA155550.0131.898823
ACAAGAT19700.0131.461245
AACAATA66900.0131.080784
ACAATTG24400.0130.609665
ACAATAG19700.0130.365725
ACAATAT22650.0129.90225
Produced by FastQC (version 0.11.5)