FastQCFastQC Report
Sat 18 Mar 2023
SRR4895562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636299
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG33580.5277393175221083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31410.4936358535845569No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC20250.31824661047714986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT12210.19189091920622223No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA9700.1524440553890545No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC8950.14065714388990083No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCACGCTT7930.12462694425105178No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC7340.11535457387171755No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA7110.11173992101197708No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT6780.10655367995234945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGCGGTGGCTCAC6400.10058164479277824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137300.0138.411358
GTGGTAT137300.0138.411356
AAGCAGT137500.0138.24461
TGGTATC138200.0137.562047
GTATCAA138600.0137.268869
CAGTGGT139500.0136.228524
AGTGGTA139400.0136.2235
AGCAGTG140800.0135.00452
GCAGTGG142550.0133.246113
CGTGGTA350.003673672761.677545
GCGAGTC803.9025363E-544.973218
TCAACGC137450.028.17557510-14
ATCAACG137300.028.11202210-14
CAACGCA138050.028.07396910-14
AGAGTAC137450.028.05125615-19
CAGAGTA138100.027.91922615-19
TACATGG138550.027.79573220-24
GTACATG138700.027.7656720-24
GCAGAGT140200.027.5318315-19
CGCAGAG140400.027.45160715-19
Produced by FastQC (version 0.11.5)