FastQCFastQC Report
Sat 18 Mar 2023
SRR4895561_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895561_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903121
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGAG905700.0143.512481
GAAGAGA911700.0142.568012
AAGAGAT298500.0141.008563
AAGAGAC128650.0137.716663
AAGAGAG281900.0137.339483
AGAGACT32000.0132.681464
AGAGACG36700.0130.591934
AAGAGAA241000.0130.518463
AGAGATA90600.0129.628394
GAGATAG31050.0129.556265
GAGACGT10950.0128.810175
AGAGATG106700.0128.683294
AGAGAGT87600.0128.317264
AGAGATT91100.0127.495054
AGAGACC42000.0126.791634
GAGATTG33050.0125.8532945
GAGATGT37700.0125.791785
AGAGATC39850.0125.506044
AGAGAAT84450.0124.7525944
GAGACTA8800.0124.2994165
Produced by FastQC (version 0.11.5)