FastQCFastQC Report
Sat 18 Mar 2023
SRR4895561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences903121
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39090.4328323668699986No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG21310.23595952258888894No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC13610.15069962939628243No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC12920.14305945714915277No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT12120.134201286427843No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG9730.10773750139793006No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA9390.1039727788413734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164050.0138.906078
AAGCAGT166150.0137.522171
GTGGTAT165850.0137.39856
TGGTATC166600.0136.866357
GTATCAA166850.0136.79079
AGTGGTA166950.0136.493215
CAGTGGT167800.0135.801794
AGCAGTG170100.0134.159332
GCAGTGG171900.0132.83833
CGTCGTA350.003670397261.6935426
GCGTACG200.006295961328.790321130-134
ATCAACG164150.028.33430910-14
TCAACGC164950.028.26670510-14
AGAGTAC165700.028.06057415-19
TACATGG166350.028.02512220-24
CAGAGTA166400.027.96848315-19
GTACATG166850.027.90662220-24
AGTACAT167900.027.68065320-24
CAACGCA169150.027.5648410-14
GAGTACA165550.027.53437420-24
Produced by FastQC (version 0.11.5)