FastQCFastQC Report
Sat 18 Mar 2023
SRR4895560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486328
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79000.5315112142138209No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC64160.43166784182226264No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG52150.35086468128165516No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC17760.11948910334730961No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG15910.10704232174863153No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT15010.10098713070062597No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT261850.0138.604811
GGTATCA264200.0137.21688
GTGGTAT264450.0137.032646
TGGTATC265650.0136.49497
GTATCAA267000.0135.643049
CAGTGGT268000.0135.405464
AGCAGTG269100.0134.790312
AGTGGTA270600.0133.971455
GCAGTGG274050.0132.487033
ATCAACG263050.028.21452310-14
TCAACGC263650.028.2049110-14
AGAGTAC263750.028.15601315-19
TACATGG264500.028.03172520-24
CAACGCA266050.027.99376310-14
GTACATG264700.027.98878720-24
CAGAGTA265400.027.96469715-19
AGTACAT266600.027.78931620-24
CGCAGAG268750.027.62682315-19
GCAGAGT268650.027.61031715-19
GAGTACA264550.027.28086720-24
Produced by FastQC (version 0.11.5)