FastQCFastQC Report
Sat 18 Mar 2023
SRR4895559_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895559_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559683
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTA1561700.0143.703612
CGTCTAG463400.0143.47333
CGTCTAA389250.0143.356493
CGTCTAT492750.0143.352283
TCCGTCT1566000.0143.309041
GTCTATG159050.0142.761644
CGTCTAC220800.0142.654313
GTCTATA133850.0142.494964
GTCTAGT153400.0142.438584
GTCTAAG125850.0142.177464
GTCTACG58000.0141.783724
GTCTAGA121850.0141.766884
GTCTAGG148450.0141.517724
GTCTACA47750.0141.331074
GTCTAAT133950.0140.777254
TCTAGTA46850.0139.746195
TCTAGAG38300.0139.008425
GTCTACT54750.0138.898994
TCTATAG44350.0138.701345
GTCTATC59900.0138.607644
Produced by FastQC (version 0.11.5)