FastQCFastQC Report
Sat 18 Mar 2023
SRR4895558_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895558_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458292
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAGC1459800.0143.768941
ATCAGCA1459800.0143.768942
TCAGCAT451400.0143.459463
TCAGCAG453250.0143.239063
TCAGCAC193900.0142.734163
TCAGCAA369300.0142.2073
CAGCATA127250.0141.942494
CAGCAGT144400.0141.579534
CAGCATG143750.0141.418784
CAGCAAT121000.0141.304984
AGCATAG44150.0140.824925
CAGCATT139900.0140.218264
AGCATGA40550.0140.195055
CAGCAAG122550.0139.576494
AGCAATG41550.0139.24565
AGCAGAT38950.0139.11825
AGCATAT39550.0138.463295
AGCAGGT54150.0138.34035
AGCATGT46900.0138.091235
CAGCAGA115950.0137.65354
Produced by FastQC (version 0.11.5)