FastQCFastQC Report
Sat 18 Mar 2023
SRR4895558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458292
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG85410.5856851714197157No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA83670.5737534046679266No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC61880.4243320267820162No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG29480.20215430105904714No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCAAGCCT21720.14894136428095334No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC21120.14482696195275022No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA16870.1156832787946447No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT16750.11486039832900408No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC15520.10642587355618766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT283650.0137.732181
GGTATCA284550.0136.895348
GTGGTAT285600.0136.366846
TGGTATC286500.0136.064097
GTATCAA287100.0135.729579
CAGTGGT292100.0133.6284
AGTGGTA292400.0133.220145
AGCAGTG293900.0132.806172
GCAGTGG297300.0131.190493
TCAACGC285800.027.96951310-14
ATCAACG284900.027.96186610-14
AGAGTAC286700.027.82260515-19
CAACGCA288700.027.7384210-14
CAGAGTA287850.027.70644815-19
TACATGG288350.027.58306520-24
GTACATG288700.027.53466420-24
AGTACAT289700.027.3899220-24
CGCAGAG291850.027.30698215-19
GCAGAGT291900.027.30230315-19
GAGTACA285800.027.15409720-24
Produced by FastQC (version 0.11.5)