FastQCFastQC Report
Sat 18 Mar 2023
SRR4895557_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895557_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3937979
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACAT3945250.0143.643431
CCACATA3948100.0143.539732
CACATAG1196350.0143.36843
CACATAT1224750.0143.022583
ACATAGT377100.0142.287814
CACATAA994850.0142.108763
CACATAC554350.0141.871843
ACATATG396700.0141.82384
ACATAGG399450.0141.441864
ACATAGA314750.0141.189254
ACATACG151750.0140.969884
ACATAAG326200.0140.931814
CATAGAG105650.0139.959525
ACATAGC121050.0139.80824
CATATGG142100.0139.808145
CATAGTA112950.0139.705185
ACATATA345550.0139.536824
CATAGAT104650.0139.096685
ACATAAT330550.0139.033634
CATAGGT131150.0138.917045
Produced by FastQC (version 0.11.5)