FastQCFastQC Report
Sat 18 Mar 2023
SRR4895556_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895556_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586518
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATG588050.0143.524871
AGGATGA588800.0143.342062
GGATGAT201050.0142.436863
GGATGAG156200.0142.384063
GGATGAC81100.0142.30723
GGATGAA155000.0141.815253
GATGACC22950.0139.201424
GATGACG21000.0139.107384
GATGACT21650.0138.919044
GATGAGA42900.0137.866244
GATGAAT57650.0137.663744
GATGATA60900.0137.524194
GATGATG62500.0137.457284
GATGAAC12100.0137.363084
ATGACGT6450.0137.210975
GATGAAG48300.0136.604694
ATGACCG5650.0136.263265
GATGAGG50950.0135.713354
ATGATGA16350.0135.102695
GATGATC22750.0135.048544
Produced by FastQC (version 0.11.5)