FastQCFastQC Report
Sat 18 Mar 2023
SRR4895556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586518
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28340.4831906267156337No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT11710.19965286657868983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA9370.1597563928131788No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT8180.13946716042815396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103000.0138.068228
GTATCAA103350.0137.461369
GTGGTAT103950.0136.875646
AGTGGTA104200.0136.478185
AAGCAGT104450.0136.428011
TGGTATC104550.0136.158977
CAGTGGT105800.0134.822374
AGCAGTG106800.0133.291242
GCAGTGG108300.0131.644563
TCGTACG255.3824706E-428.78799250-54
TCAACGC103450.028.11964210-14
ATCAACG103200.028.04828610-14
AGAGTAC103350.028.0368715-19
CAGAGTA104450.027.79672615-19
TACATGG104450.027.76201220-24
GTACATG104900.027.6703720-24
CAACGCA105550.027.61472710-14
AGTACAT104950.027.58859320-24
GCAGAGT105650.027.44012815-19
GAGTACA103450.027.39015620-24
Produced by FastQC (version 0.11.5)