FastQCFastQC Report
Sat 18 Mar 2023
SRR4895555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330760
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101880.4371106420223447No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG91930.39442070397638535No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT85740.36786284302116046No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA57030.24468413736292027No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC48550.20830115498807256No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC46020.19744632652010505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT34020.14596097410286774No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC28910.12403679486519417No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA28860.1238222725634557No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGCGGTGGCTCA25670.11013574971254013No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAT24220.10391460296212394No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC24080.10331394051725618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT531850.0138.859621
GGTATCA535450.0138.055288
GTGGTAT535700.0137.990846
GTATCAA537550.0137.529339
TGGTATC538250.0137.377217
AGTGGTA538100.0137.335275
CAGTGGT542250.0136.37714
AGCAGTG544850.0135.546462
GCAGTGG554850.0133.11653
CGTGGTA1851.754288E-731.1227135
TCAACGC533750.028.25993510-14
ATCAACG533550.028.20038210-14
AGAGTAC536800.028.02196515-19
CAGAGTA538900.027.91276715-19
CAACGCA540800.027.91016210-14
TACATGG538800.027.9023220-24
GTACATG542250.027.71948420-24
GCAGAGT543900.027.6244115-19
AGTACAT544850.027.54228820-24
AACGCAG548000.027.53820810-14
Produced by FastQC (version 0.11.5)