FastQCFastQC Report
Sat 18 Mar 2023
SRR4895554_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895554_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187858
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCA1191350.0143.57182
CTGTCAG402950.0143.440933
GTCTGTC1193450.0143.319151
CTGTCAT432450.0143.278933
CTGTCAA356800.0143.187683
TGTCAAG123700.0142.074114
GTCATAG44750.0141.58155
TGTCATG157200.0141.475834
TGTCAGA115550.0141.377874
TGTCAAT131300.0141.307944
TGTCAGG148350.0141.179964
TGTCATA128800.0141.088094
TGTCAGT144300.0141.051064
GTCATAA39400.0140.705615
GTCAATG47300.0140.64615
GTCATGA44900.0140.627465
GTCATTA45900.0140.230265
GTCAGGA43000.0140.14385
GTCAGAT44400.0139.778765
GTCAGAG37400.0139.567175
Produced by FastQC (version 0.11.5)