FastQCFastQC Report
Sat 18 Mar 2023
SRR4895554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187858
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59710.5026695110021568No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG35780.3012144549264306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC33620.2830304632371883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGCGGTGGCTCACGCCT32370.2725073198985064No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA21090.17754647441024096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT20890.17586277147605187No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC16020.13486460502854716No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT13680.11516528069853468No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA13230.1113769490966092No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC12700.10691513632100806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT237800.0139.376451
GGTATCA238250.0138.829518
GTATCAA240050.0138.238429
GTGGTAT240050.0137.698536
TGGTATC240350.0137.586587
AGCAGTG242250.0136.34062
CAGTGGT243000.0135.967624
AGTGGTA243700.0135.606635
GCAGTGG246600.0133.906393
ATCAACG238650.028.33481410-14
CAACGCA240450.028.23648610-14
TCAACGC240150.028.21779410-14
AGAGTAC239850.028.1870515-19
CAGAGTA240400.028.1105815-19
TACATGG238350.028.06840920-24
GTACATG239750.027.9885920-24
AGTACAT241950.027.84122320-24
CGCAGAG242950.027.82145915-19
AACGCAG246750.027.59725410-14
GCAGAGT244600.027.58668915-19
Produced by FastQC (version 0.11.5)