FastQCFastQC Report
Sat 18 Mar 2023
SRR4895553_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895553_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2002582
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGA2004250.0143.872992
GTCGTAG2004800.0143.833531
CGTAGAT743650.0143.753083
CGTAGAA594300.0143.704363
CGTAGAG666800.0143.498443
GTAGATG256900.0141.136524
GTAGAAG205100.0141.081534
GTAGATA227650.0141.053864
GTAGAGT237600.0139.874054
GTAGAGG242150.0139.683914
GTAGAGA201050.0139.590414
GTAGAAT230850.0139.37934
TAGAGTA75300.0138.927585
GTAGAAA174150.0138.165864
TAGAGGA69600.0136.650565
GTAGATT280550.0136.450214
TAGATGG93600.0136.380025
TAGAATG79500.0135.844475
TAGATAG80800.0135.530085
TAGATGA76850.0135.282355
Produced by FastQC (version 0.11.5)