FastQCFastQC Report
Sat 18 Mar 2023
SRR4895552_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895552_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729839
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA730150.0143.926861
TACGCAA730650.0143.828372
ACGCAAT265750.0143.589513
CGCAATA79200.0143.450454
ACGCAAG255600.0143.319843
CGCAATG92100.0143.135974
CGCAAGT92850.0142.910314
ACGCAAA210800.0142.902953
CGCAATT94700.0142.855484
CGCAAGG91700.0142.661134
CGCAAAG79250.0142.451464
CGCAAGA71200.0142.17574
GCAATTA29350.0141.788125
GCAATGA25150.0141.419425
GCAATTG32750.0141.35785
GCAAGTA26500.0141.00735
GCAATAT28050.0140.915765
GCAAGGT33400.0140.546895
GCAATGT35350.0140.329795
GCAATAG28950.0140.265415
Produced by FastQC (version 0.11.5)