FastQCFastQC Report
Sat 18 Mar 2023
SRR4895552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729839
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48110.6591864781136662No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG33220.4551688797118269No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC21900.3000661789791995No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCC13080.17921760826702876No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA12930.17716236046580136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT12710.1741479970240012No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC9760.13372812359986244No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT8060.11043531518595197No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA7860.10769498478431544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100450.0134.17828
GTGGTAT100700.0134.059596
GTATCAA101250.0133.189159
AAGCAGT101250.0133.118041
TGGTATC101550.0132.72487
AGCAGTG102600.0131.226142
AGTGGTA103600.0130.306965
CAGTGGT103900.0130.04
GCAGTGG105000.0128.295243
AGAGTAC100200.027.53492415-19
TACATGG99350.027.48062120-24
ATCAACG100050.027.4466710-14
ACGTATG1050.00797237627.4281659
CAGAGTA100450.027.39471815-19
TCAACGC100850.027.3717310-14
CAACGCA101600.027.2263710-14
GTACATG100650.027.13998620-24
GCAGAGT101500.027.01201415-19
AGTACAT102200.026.82700220-24
CGCAGAG102400.026.78866815-19
Produced by FastQC (version 0.11.5)