FastQCFastQC Report
Sat 18 Mar 2023
SRR4895551_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895551_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516540
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA1518050.0143.845372
TGCGAAT562100.0143.800263
TGCGAAG525950.0143.663853
GGTGCGA1521200.0143.54751
GCGAATA165550.0143.427024
TGCGAAA430350.0143.406853
GCGAAGT186100.0143.296024
GCGAATG194050.0143.176154
GCGAAAT163000.0143.064834
GCGAATT202900.0142.998864
GCGAAAG160600.0142.692324
GCGAAGA153650.0142.492954
CGAATTA59000.0142.161975
CGAATAG58750.0142.154195
CGAAGTA58750.0142.154195
CGAAGAT57850.0142.125585
GCGAAGG188300.0142.080644
CGAATGG69200.0141.911585
CGAAATG56250.0141.81655
CGAAGTT67800.0141.76245
Produced by FastQC (version 0.11.5)