FastQCFastQC Report
Sat 18 Mar 2023
SRR4895551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516540
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101980.6724517652023685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC48090.3171034064383399No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG44890.29600274308623575No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC25570.16860748809790707No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA20190.133131997837182No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT20080.13240666253445343No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT19250.1269336779775014No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC19200.12660398011262478No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19160.1263402218207235No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCAGTGGCTCA18720.12343888060980918No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA16770.1105806638796207No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA16480.10866841626333629No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15710.10359106914423623No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG15480.10207445896580374No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA215550.0137.474698
AAGCAGT216450.0137.149461
GTGGTAT216350.0136.999626
GTATCAA217150.0136.693839
TGGTATC219250.0135.089047
CAGTGGT221350.0133.904984
AGTGGTA221500.0133.846825
AGCAGTG223000.0132.862782
GCAGTGG231600.0127.9871443
TCGTACG700.028.80152950-54
ATCAACG214650.028.19381710-14
TCAACGC215700.028.0632510-14
AGAGTAC215500.028.04307615-19
TACATGG213900.027.91118820-24
GTACATG215550.027.85118120-24
CAGAGTA217650.027.74621615-19
CAACGCA219800.027.60528410-14
AGTACAT218500.027.59378620-24
GAGTACA215150.027.4679220-24
GCAGAGT221600.027.18016615-19
Produced by FastQC (version 0.11.5)