FastQCFastQC Report
Sat 18 Mar 2023
SRR4895550_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895550_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567655
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAC1571250.0143.664541
GAGAACA1572450.0143.55492
AGAACAT555050.0143.086463
AGAACAG563500.0142.678443
GAACAGT204950.0142.483894
GAACATG196300.0142.380664
GAACATA167850.0141.892654
GAACAAT171700.0141.855914
AGAACAA462800.0141.536453
GAACATT193900.0141.358144
GAACAGA160800.0141.039344
GAACAAG172600.0140.615664
GAACAGG201150.0140.594134
AACATAG62650.0138.937975
AACAGTG69600.0138.615365
AACATGG74750.0138.022775
AACATTG72400.0137.630075
ACATAGG24550.0136.076226
AACAGGT71650.0135.85525
AACATGT72750.0135.780335
Produced by FastQC (version 0.11.5)