FastQCFastQC Report
Sat 18 Mar 2023
SRR4895549_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895549_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147659
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA2149250.0143.888242
TCGGTAT761350.0143.654983
GCTCGGT2154450.0143.540951
TCGGTAG744200.0143.51173
TCGGTAA645800.0143.4383
CGGTATG271950.0143.174734
CGGTATA225350.0143.132814
CGGTAAG223800.0142.998154
CGGTAAT233900.0142.979644
CGGTAGA214650.0142.955644
CGGTAGT263400.0142.76544
CGGTATT265600.0142.748494
CGGTAGG269300.0141.856634
CGGTAAA190450.0141.689394
GGTATGA79750.0136.682825
GGTATAG80600.0135.509355
GGTAGTA86950.0134.969855
GGTATTA83150.0134.730555
GGTAGAG80750.0134.63355
GGTAATA74550.0134.434395
Produced by FastQC (version 0.11.5)