FastQCFastQC Report
Sat 18 Mar 2023
SRR4895549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147659
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA126180.587523438311203No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC43080.20059050342722007No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG42900.19975238154660493No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC37870.17633153121608225No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT29310.13647417956016294No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC29200.1359619939664537No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA29070.13535668371934279No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA24480.11398457576365709No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT23020.1071864760653344No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA22660.10551023230410414No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA22230.103508052255968No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT22050.1026699303753529No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG21570.10043493869371255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA272100.0136.292248
GTGGTAT273250.0135.718646
AAGCAGT274350.0135.603841
TGGTATC274100.0135.27157
GTATCAA275350.0134.971189
AGTGGTA280150.0132.427345
AGCAGTG282350.0131.506682
CAGTGGT282350.0131.44654
GCAGTGG288150.0128.731743
ATCAACG270950.028.08626410-14
TCAACGC272000.028.00960710-14
AGAGTAC271450.027.99222615-19
CAGAGTA272300.027.89955715-19
CAACGCA275250.027.72073410-14
TACATGG271900.027.70357920-24
GTACATG273050.027.63436520-24
AGTACAT276400.027.3723720-24
GAGTACA272250.027.17077420-24
CGCAGAG279950.027.14230715-19
GCAGAGT280900.027.00437715-19
Produced by FastQC (version 0.11.5)