FastQCFastQC Report
Sat 18 Mar 2023
SRR4895547_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895547_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724098
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGA174450.0143.666754
CTATCAG175100.0143.544624
CTCTATC1729600.0143.534872
CCTCTAT1730600.0143.451921
TCTATCG611100.0143.431413
CTATCGG211050.0143.041724
TCTATCA505850.0142.82983
CTATCGT225400.0142.655334
CTATCTG213800.0142.548874
TATCAGG60200.0142.083345
TATCGAT63450.0142.067895
TATCGAG58650.0141.915
ATCGATA19000.0141.723286
CTATCAA146650.0141.689424
CTATCTA176800.0141.67574
TCTATCT621050.0141.655143
CTATCAT184900.0141.621634
TATCGGA61000.0141.400255
TATCGTA69800.0140.902445
TATCGAA54800.0140.84375
Produced by FastQC (version 0.11.5)