FastQCFastQC Report
Sat 18 Mar 2023
SRR4895547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1724098
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101310.5876116090848664No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG50130.29076073401859986No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC34850.20213468143922214No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC34240.19859659949724434No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT29300.1699439359015555No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC27510.15956169544886661No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA27010.15666162828331104No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA26690.1548055852973555No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA23050.13369309633211104No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA22070.12800896468762218No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT22050.12789296200099992No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT21450.12441288140233328No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG20990.12174481961002216No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA20730.12023678468393328No Hit
GGCAGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCG20340.11797473229479993No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCGGGCGCGGTGGCTCACG20330.11791673095148884No Hit
GTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAG20210.1172207148317555No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCACTGCGTGGCCCTGTGTA20160.11693070811519994No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA20030.11617669065215551No Hit
CACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAG19550.11339262617322218No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA19220.11147858184395551No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC19140.11101457109746662No Hit
GAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTC18610.10794049990197772No Hit
AGACGCTTTCAGTGGGTTGACGGCAGCCGCTGGAACTTTGCGTACTGGGC18500.10730248512555551No Hit
GCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGT18390.10666447034913329No Hit
CGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGG18320.10625846094595552No Hit
AGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGC18030.10457642198993329No Hit
CAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGT17920.10393840721351105No Hit
ACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCCGAGGA17730.10283638169059994No Hit
AGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCAG17710.10272037900397772No Hit
CGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCAT17640.10231436960079995No Hit
GTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCC17500.1015023507944444No Hit
AGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTC17420.1010383400479555No Hit
TGCTCACCAGCCCTGGTCCCGCGGTGGTCACTGCGTGGCCCTGTGTACCC17380.10080633467471106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA242700.0137.917768
AAGCAGT244000.0137.659071
GTGGTAT243700.0137.440466
TGGTATC245750.0136.235357
GTATCAA246950.0136.039839
AGCAGTG246750.0135.657992
AGTGGTA247600.0135.333775
CAGTGGT248100.0134.944934
GCAGTGG255600.0130.989093
ATCAACG243500.028.21440110-14
TCAACGC244900.028.12955310-14
AGAGTAC244250.028.08649815-19
CAGAGTA246050.027.90443815-19
CAACGCA248300.027.79076610-14
TACATGG245050.027.78325820-24
GTACATG245750.027.72169720-24
AGTACAT248350.027.57063520-24
GCAGAGT248250.027.5701415-19
GAGTACA244750.027.29956220-24
CGCAGAG251550.027.26570115-19
Produced by FastQC (version 0.11.5)