FastQCFastQC Report
Sat 18 Mar 2023
SRR4895546_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895546_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269495
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATC1271850.0143.724461
CTAATCC1272100.0143.696212
TAATCCG446150.0143.657293
AATCCGG157400.0143.447274
AATCCGA130900.0143.446154
AATCCGT157600.0142.945434
ATCCGAG43950.0142.849465
TAATCCA380550.0142.747483
TAATCCT448250.0142.695163
ATCCGAT49250.0142.680485
ATCCGTA47350.0142.171515
AATCCTG157950.0142.127294
ATCCGAA38450.0141.936425
ATCCGGA43900.0141.864125
ATCCGGT59000.0141.799625
AATCCAG137000.0141.736394
AATCCAT134350.0141.20954
AATCCTA130600.0140.908974
ATCCGGG56400.0140.42185
ATCCTAG46950.0140.315775
Produced by FastQC (version 0.11.5)