FastQCFastQC Report
Sat 18 Mar 2023
SRR4895546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269495
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG63540.5005139839069866No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57030.4492337504283199No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC46700.3678628115904356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT21770.17148551195554138No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19380.15265912823603087No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGGGGAGGTAGTGACGAAAA15110.11902370627690538No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC14370.11319461675705694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT207900.0135.482181
GGTATCA207850.0135.370888
GTGGTAT209000.0134.419336
TGGTATC210000.0133.984947
GTATCAA210750.0133.883919
CAGTGGT212250.0132.361084
AGCAGTG213000.0131.832612
AGTGGTA213450.0131.616965
GCAGTGG219950.0127.765163
ATCAACG207500.027.79992310-14
AGAGTAC208550.027.63924215-19
GTACATG207450.027.60619720-24
TCAACGC209700.027.5906710-14
CAACGCA210500.027.52685410-14
CAGAGTA209300.027.51956215-19
TACATGG208500.027.4671720-24
AGTACAT209850.027.39340420-24
ACATGGG210900.026.98390220-24
CGCAGAG213750.026.9601115-19
GAGTACA208950.026.84979820-24
Produced by FastQC (version 0.11.5)