FastQCFastQC Report
Sat 18 Mar 2023
SRR4895545_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895545_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914263
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAC1917900.0143.71972
CCGACAA1918200.0143.697221
GACAACT673800.0143.416733
GACAACG690000.0143.336363
GACAACA560050.0142.322533
ACAACGT248300.0142.084
ACAACTG236700.0141.834144
ACAACGG246650.0141.775284
ACAACGA200050.0141.330524
CAACGTA76500.0141.076225
CAACTAG73750.0139.99125
CAACGGT90100.0139.918445
CAACGTG83900.0139.874735
CAACGAT75250.0139.783955
ACAACTA208700.0139.509034
CAACGGA76100.0139.357935
CAACTGA64450.0139.07855
CAACGAG69500.0138.91775
CAACTGG88250.0138.527685
CAACGTT91850.0138.506765
Produced by FastQC (version 0.11.5)