FastQCFastQC Report
Sat 18 Mar 2023
SRR4895545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914263
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA103430.5403123813185544No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG42300.2209727712440767No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC37210.19438290349863108No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT22470.11738198983107337No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC21510.11236700495177517No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA19640.10259823232230891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA273550.0136.758778
GTGGTAT274250.0136.435966
GTATCAA276550.0135.743859
TGGTATC276350.0135.321037
AAGCAGT279200.0134.436691
AGTGGTA281800.0132.703925
CAGTGGT281850.0132.62934
AGCAGTG286350.0130.677572
GCAGTGG290550.0128.63993
AGAGTAC272250.028.10148415-19
TCAACGC274150.028.0272410-14
ATCAACG274250.027.9960210-14
TACATGG272100.027.90573120-24
CAACGCA276250.027.88194810-14
CAGAGTA274950.027.84647815-19
GTACATG275100.027.62758620-24
AGTACAT278200.027.45948820-24
GAGTACA272700.027.35324120-24
GCAGAGT279850.027.33317815-19
CGCAGAG281450.027.23918715-19
Produced by FastQC (version 0.11.5)