FastQCFastQC Report
Sat 18 Mar 2023
SRR4895544_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895544_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777787
Sequences flagged as poor quality0
Sequence length150
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTCG282500.0143.481813
CCATCCT781750.0143.254751
ATCCTCA225900.0143.162873
CATCCTC782550.0143.108292
TCCTCGA81200.0141.952224
ATCCTCT274550.0141.84123
TCCTCGG106700.0140.617774
CCTCGGA30300.0139.952155
CTCGAAT8500.0139.765466
TCCTCAG84400.0139.726364
TCGGATA3200.0139.500757
CCTCGTA31400.0139.176515
TCCTCTA84050.0138.68074
CCTCGAT29050.0138.539155
CCTCTAG31200.0138.453385
TCCTCGT100450.0138.185974
CTCGTAA9600.0138.000736
CCTCGAA25050.0137.668535
CCTCAGA23500.0137.557855
CCTCGAG29400.0137.379675
Produced by FastQC (version 0.11.5)