FastQCFastQC Report
Sat 18 Mar 2023
SRR4895544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777787
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG53840.6922203636728307No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC39300.5052797231118545No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35290.45372319156787144No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGCGGTGGCTCA20730.2665254111986958No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCACGCCTCCTCCAAGTCC16330.20995465339482405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGCGGTGGCTCAC9910.12741277496281117No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9410.12098427975782573No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTCGGCCGGACGCGGTGG9400.12085570985372603No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC9200.11828431177173186No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCGGGCGCGGTGGCTCA8750.11249866608724496No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACCACGCCTCCTCCAAGTC8560.1100558379093505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT8190.1052987514576613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT126950.0134.302761
GGTATCA128100.0132.807538
GTGGTAT128700.0132.308756
TGGTATC129550.0131.162777
GTATCAA130300.0130.786189
AGCAGTG130300.0130.573562
CAGTGGT131000.0130.040734
AGTGGTA133850.0127.2180565
GCAGTGG135950.0125.2529453
CAACGCA128550.027.28621310-14
AGAGTAC128350.027.26212115-19
GTACATG127750.027.19006720-24
TACATGG127750.027.19006720-24
CAGAGTA128800.027.14451415-19
ATCAACG128300.027.13736710-14
TCAACGC129300.027.03884910-14
AGTACAT129400.026.84336320-24
GCAGAGT130650.026.60584415-19
GAGTACA127900.026.56141920-24
CGCAGAG131150.026.5593115-19
Produced by FastQC (version 0.11.5)