FastQCFastQC Report
Sat 18 Mar 2023
SRR4895543_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895543_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543647
Sequences flagged as poor quality0
Sequence length150
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAC2549200.0143.676992
CCTTACG847200.0143.51743
CACCTTA2555950.0143.297551
CTTACGA235800.0143.229974
CTTACGG287150.0142.965324
CTTACGT324450.0142.772834
CTTACAG250300.0142.641394
CCTTACA763500.0142.607123
CTTACTG310600.0142.185264
CCTTACT951350.0142.184593
CTTACTA284100.0141.737854
TTACGGA82750.0141.383155
TTACGAG73200.0141.042625
TACGGAT34750.0140.683886
TTACGAT95450.0140.221865
TTACGTA100150.0139.967535
TACGTAG31600.0139.897726
CTTACAA232900.0139.418154
TTACGGT117350.0139.391895
TTACGTG106100.0139.107575
Produced by FastQC (version 0.11.5)