FastQCFastQC Report
Sat 18 Mar 2023
SRR4895543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543647
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162780.6399472882833191No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG139360.5478747640690709No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC120420.47341474662168137No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC49940.19633227409306403No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT39180.15403080694766216No Hit
CCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCA38230.15029601198593986No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC37950.1491952303130112No Hit
CTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCA32080.12611812881268508No Hit
AGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTT31240.12281578379389907No Hit
TGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGGTCA30940.12163637485861835No Hit
GAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCTGGT30660.12053559318568967No Hit
AGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGG30170.11860922525806451No Hit
AAGACTTCCTTTCATCTGTTCCTACTGAGCTGGTCCCAGCCAGCAGTTCA29890.11750844358513583No Hit
GTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAG28100.11047130360462754No Hit
GGCAGCCGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCG27590.1084663084146503No Hit
CGCTGGAACTTTGCGTACTGGGCTGCTCACCAGCCCTGGTCCCGCGGTGG25960.10205818653295838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT320200.0137.69876
GGTATCA320850.0137.419758
AAGCAGT322650.0137.196751
TGGTATC324050.0135.996087
GTATCAA326900.0135.383069
CAGTGGT327500.0134.73934
AGCAGTG330400.0133.477392
AGTGGTA331650.0133.031595
GCAGTGG334100.0131.977633
CTCGGTA950.004890938330.3164252
ATCAACG321750.028.08732810-14
AGAGTAC320950.028.0771315-19
TCAACGC322850.028.0496110-14
CAACGCA325400.027.88732510-14
CAGAGTA324200.027.80899215-19
GTACATG322450.027.78347220-24
TACATGG322350.027.75188320-24
AGTACAT327200.027.49016820-24
CGCAGAG329300.027.40016715-19
GCAGAGT329350.027.34791215-19
Produced by FastQC (version 0.11.5)