FastQCFastQC Report
Sat 18 Mar 2023
SRR4895542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89850
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT25192.8035614913745133No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA24812.7612687813021703No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAAC7080.7879799666110184No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCCTGGAGATTGTCT4930.5486922648859209No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTCTTGCTTCAACA4670.5197551474680022No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCATCTCCTCTAAC4000.4451864218141347No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCATCTCCTCTAACT3600.40066777963272115No Hit
ATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTAT2520.28046744574290483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCCTGGAGATTGTC2490.27712854757929883No Hit
TATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAA2170.24151363383416807No Hit
ACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTA2140.23817473567056205No Hit
ATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTG2050.228158041179744No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGAATTCTGGACATTAAT2040.2270450751252087No Hit
AAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATAT2010.22370617696160267No Hit
AAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATA1990.22148024485253198No Hit
AAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCT1910.2125765164162493No Hit
ATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCA1870.20812465219810797No Hit
TTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAA1830.20367278797996663No Hit
ATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGC1760.19588202559821924No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC1680.18697829716193656No Hit
ATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGAC1650.18363939899833057No Hit
ATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAA1650.18363939899833057No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1640.18252643294379522No Hit
ATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCA1600.17807456872565386No Hit
AACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGA1590.17696160267111852No Hit
AAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACA1560.17362270450751252No Hit
AGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATT1560.17362270450751252No Hit
TAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATAT1550.17250973845297718No Hit
ATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCAT1540.17139677239844184No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA1530.17028380634390652No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC1490.16583194212576516No Hit
AATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCC1490.16583194212576516No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG1470.1636060100166945No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1450.16138007790762382No Hit
TATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTA1440.16026711185308848No Hit
TAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAG1430.15915414579855314No Hit
GGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT1420.1580411797440178No Hit
ATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTA1420.1580411797440178No Hit
AAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTC1420.1580411797440178No Hit
TAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAA1420.1580411797440178No Hit
CACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTA1410.15692821368948245No Hit
ACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAA1400.15581524763494714No Hit
AGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAA1400.15581524763494714No Hit
AAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGC1400.15581524763494714No Hit
TTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCT1390.1547022815804118No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC1360.15136338341680577No Hit
AACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACAT1360.15136338341680577No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA1350.15025041736227046No Hit
CTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAA1330.14802448525319978No Hit
CATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTA1320.14691151919866444No Hit
CCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACAT1300.14468558708959378No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT1300.14468558708959378No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT1290.14357262103505844No Hit
CTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATA1280.14245965498052307No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG1260.14023372287145242No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC1250.13912075681691707No Hit
AAAATGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCT1250.13912075681691707No Hit
TATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCA1250.13912075681691707No Hit
AGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCC1250.13912075681691707No Hit
TATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGT1240.13800779076238173No Hit
TATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAG1230.13689482470784642No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT1200.1335559265442404No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT1200.1335559265442404No Hit
CATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGA1200.1335559265442404No Hit
CCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTA1190.13244296048970505No Hit
GAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTC1180.13132999443516974No Hit
AGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAA1180.13132999443516974No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT1170.1302170283806344No Hit
GATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAAT1160.12910406232609906No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCATCGACAATAAAC1160.12910406232609906No Hit
ATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGA1160.12910406232609906No Hit
TAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAA1150.12799109627156371No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT1150.12799109627156371No Hit
AACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCA1150.12799109627156371No Hit
TCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGC1140.1268781302170284No Hit
TAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAAC1140.1268781302170284No Hit
GGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATT1130.12576516416249306No Hit
TTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGAT1130.12576516416249306No Hit
AAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAA1120.12465219810795772No Hit
CCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTAT1110.12353923205342236No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG1110.12353923205342236No Hit
TGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTA1100.12242626599888702No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA1090.1213132999443517No Hit
TTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAG1080.12020033388981635No Hit
TTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAAC1080.12020033388981635No Hit
TCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTAT1070.11908736783528102No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG1050.11686143572621034No Hit
ACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATC1040.11574846967167501No Hit
GGCTGCTTGAGGAGCTTGAAGCAGATGATATCCCGATGCTGACGGCTTAC1040.11574846967167501No Hit
TTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCAC1040.11574846967167501No Hit
AGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATA1030.11463550361713967No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG1030.11463550361713967No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA1030.11463550361713967No Hit
ATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATG1030.11463550361713967No Hit
TGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAA1020.11352253756260434No Hit
ACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCC1020.11352253756260434No Hit
CAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATA1000.11129660545353368No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC990.11018363939899833No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT990.11018363939899833No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG980.10907067334446299No Hit
ATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTT970.10795770728992767No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA970.10795770728992767No Hit
ACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCAT960.10684474123539232No Hit
ATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTC960.10684474123539232No Hit
ATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAG950.105731775180857No Hit
TATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGG950.105731775180857No Hit
CTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTA940.10461880912632164No Hit
GTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAG930.1035058430717863No Hit
TCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATA920.10239287701725097No Hit
TACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAA910.10127991096271563No Hit
TTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGA910.10127991096271563No Hit
AGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATT900.10016694490818029No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG900.10016694490818029No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAC100.0072282134144.000023
AAGCAGT15300.0143.529421
AGTGGTA15650.0139.859425
GTGGTAT15700.0139.414026
AGCAGTG15800.0138.987352
CAGTGGT15850.0138.54894
GGTATCA15900.0138.11328
GTATCAA15900.0137.660379
GCAGTGG16100.0136.844713
TGGTATC17200.0127.674427
ATGGGTG304.6056783E-528.80000125-29
ATTAGGT200.00627969328.800001135-139
GTATTAG200.00627969328.800001135-139
GACAATA304.6056783E-528.80000140-44
ATAGAAC304.6056783E-528.80000155-59
TGTTGAT304.6056783E-528.800001105-109
TATTAGG200.00627969328.800001135-139
TCCAAGC1250.028.80000190-94
TTAGTGA200.00627969328.800001135-139
CATCGAC304.6056783E-528.80000135-39
Produced by FastQC (version 0.11.5)