FastQCFastQC Report
Sat 18 Mar 2023
SRR4895541_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895541_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127219
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCT1129300.0143.722921
TTGGCTC1133000.0143.253572
TGGCTCA326050.0143.107443
GGCTCTA120650.0143.09564
GGCTCAT120100.0142.85174
TGGCTCG380700.0142.572333
GGCTCAA98450.0142.52814
GGCTCGA107800.0142.254264
TGGCTCT429350.0142.162933
GCTCGTA43150.0141.98855
GCTCATA37250.0141.671375
GGCTCAG108050.0141.658584
GCTCGAT40350.0141.49275
GGCTCGT144100.0141.242774
GCTCTAG41300.0141.201525
GCTCAAG33050.0141.15885
GCTCGAA31300.0140.540425
GCTCAAT35050.0140.498755
GCTCTAT46900.0140.459995
GCTCATG41400.0140.164845
Produced by FastQC (version 0.11.5)