FastQCFastQC Report
Sat 18 Mar 2023
SRR4895541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127219
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG87050.7722545485837269No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC61330.5440823832813322No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54600.48437792478657654No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAATTCTGGACATTAATTA21840.1937511699146306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATT19890.17645195831511No Hit
GAGGAGGCTACTGGCGTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATC14060.12473175132782538No Hit
GGTGGTCACTGCGTGGCCCTGTGTACCCGAGGAGGCTACTGGCGTCGAGC11470.10175484976743648No Hit
GTCGAGCCCACTGCCTCAGAAGACTTCCTTTCATCTGTTCCTACTGAGCT11280.10006928556030373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT185600.0136.47141
GGTATCA186000.0135.469138
GTGGTAT186750.0135.040746
GTATCAA188350.0134.352269
AGCAGTG188900.0133.668052
TGGTATC189300.0133.107547
CAGTGGT192150.0131.395554
AGTGGTA192200.0131.286445
GCAGTGG195250.0129.136463
CAACGCA187400.027.89792610-14
AGAGTAC186650.027.8714215-19
ATCAACG187200.027.72775310-14
TCAACGC187750.027.69254310-14
CAGAGTA188000.027.64830415-19
TACATGG186800.027.63517620-24
TCGTACG1150.027.54898650-54
GTACATG187400.027.53901120-24
CGCAGAG190100.027.2822815-19
AGTACAT190050.027.2686720-24
GCAGAGT191150.027.07215915-19
Produced by FastQC (version 0.11.5)