FastQCFastQC Report
Sat 18 Mar 2023
SRR4895540_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4895540_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412189
Sequences flagged as poor quality0
Sequence length150
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATTC1415750.0143.630841
TCATTCC1417700.0143.433272
CATTCCG472850.0143.387943
ATTCCGA139900.0143.173574
ATTCCGG158100.0142.676354
CATTCCA431050.0142.610633
ATTCCGT174900.0142.473864
CATTCCT517200.0142.242983
ATTCCTG168000.0141.982764
ATTCCTA159650.0141.020544
ATTCCAT159000.0140.419714
ATTCCAG140600.0140.412434
TCCGAAT18000.0139.997076
TCCGGAT17100.0138.52346
ATTCCAA139350.0137.693574
TCCGGTA19550.0136.99976
TTCCGAG47600.0136.13165
TTCCGAT54300.0135.776175
TCCGATA17450.0135.745016
TTCCTAG57000.0135.534015
Produced by FastQC (version 0.11.5)